R2C2 full-length cDNA sequencing of hemoglobin depleted polar bear wh... - SRA

SRX5243183: R2C2 full-length cDNA sequencing of hemoglobin depleted polar bear whole blood transcriptome
1 OXFORD_NANOPORE (MinION) run: 5,018 spots, 19.2M bases, 15.1Mb downloads

Design: R2C2 protocol. Full-length cDNA generated using oligodT priming and template switching, depleted of hemoglobin transcripts using Long-DASH protocol, and circularized using Gibson Assembly, amplified using rolling circle amplification, debranched.

Submitted by: (UCSC)

Study: Hemoglobin depleted polar bear whole blood transcriptome

PRJNA514749 • SRP178553 • All experiments • All runs

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Smart-seq2 Illumina and R2C2 ONT data for polar bear transcripts. Input full-length cDNA was depleted of hemoglobin using Long-DASH method.

Sample:

SAMN10734297 • SRS4245857 • All experiments • All runs

Organism: Ursus maritimus

Library:

Name: PB19_undepleted_R2C2

Instrument: MinION

Strategy: FL-cDNA